Regulatory

Part:BBa_K284003:Design

Designed by: Tais Sineiro Herig, Raissa Estrela Curado and Gleidson Silva Teixeira   Group: iGEM09_UNICAMP-Brazil   (2009-09-26)

Partial DLD Promoter from Kluyveromyces lactis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Genes regulated by this promoter can be induced by lactate even in the presence of glucose.

Source

This part was amplified by PCR from Kluyverimyces lactis genomic DNA.

References

Lodi T, Goffrini P, Bolondi I and Ferrero I. 1998. Transcriptional regulation of the KlDLD gene, encoding the mitochondrial enzyme D-lactate ferricytochrome c oxidoreductase in Kluyveromyces lactis: effect of Klhap2 and fog mutations. Curr Genet 34, 12-20.